The KPV Masterclass | The Complete User's Guide

0:00 Hey everybody, this is Hunter Williams. I hope you're doing amazing wherever you might be in the world. Today's video is going to be the KPV masterclass. TB 500, you know, GLPs, all those out there, but KPV is so underrated. I think the amount of people that know about KP or at least know how to intelligently use KP is much fewer than there's actually out that would benefit

0:37 from this. So I'm excited for this one because I think there's probably a gap in the marketplace just of what people understand about KPV and actually using it. The reason I know is because having, you know, just seeing the commercial side of the peptide market, this is like one of least demanded, but in my opinion, it should be the most demanded peptides out there. And so I am not saying that to try to push KP by any means or anything like that. But I think it's one that is not as understood in terms of how to use it and what actually it can do, because it is so versatile and it does so many things.

1:10 Obviously, you probably have heard of the Chloe blend. KPV is a part of Chloe Blend, but by itself, it really can stand on its own for some of things that it doesn't. So that's why I'm so excited because I this is one of those ones. Everybody knows about GLPs, everyone knows, about BPC, TP500, you name it. But when it comes to some of the peptides that people don't know that much about, I would put KPV in my top five peptide all day, every day. And I think a lot of people didn't even realize what it does or how to use it, so that's why I'm so excited. We're going to cover that today, we're gonna go over everything that I could have possibly thought about from how use so by the end of this video,

1:41 however long it ends up being, You have a very good understanding of what KPVE does and how you can actually practically research with it Before I do that, as always, just make sure you are on the email list. That is the best place to stay in touch with me. The link will be in the bottom of the description of wherever this video is. And also too, if you want direct access to me, the place is Axion Collective. We do live coaching calls every Thursday night at 8 p.m. Eastern in there. I think we've got over 250 people in here now. It is awesome. There are some amazing people, amazing coaches, and amazing doctors.

2:14 So it's a really good hub place where if you wanna ask questions about peptides, you can obviously privately message me, but then also talk to some of the amazing people in there as well. And then last thing, just because I recently developed this, check out the AI chat tool I have. It's just chat.hunterwilliams.health.com. Seems to be working really well, a lot of people that want to get questions answered for me but I don't have time to just, because, I do not have the bandwidth and data just to answer questions for people who are not in my private group. make sure you check out that tool because it seems to be really well.

2:44 I put a lot of time and effort into building that and I've gotten really good feedback on it so far. So check that out and play around with it if you want. But without further ado, I'm going to share my screen and today we are going What we're going to cover is what KPV is, how it works, who benefits. And that's what I said in the intro is there's so many use cases for KPB. I think it's vastly underutilized in peptide space right now. Some dosing protocols, luckily the dosage protocols are very straightforward.

3:16 Practical use, also how to stack it with other peptides and then just what to track and what the measure objectively and subjectively when you are using it. Now, what is KPVE? It's pretty cool. It comes out of this melanocyte stimulating hormone. Family, it just has three amino acids, one of which is lysine, the next is proline, and the nex is valine. And the single letter codes for those are KPV. Hence, we have KPB. The molecular weight is under 400 daltons. It's tiny by peptide standards. So again, when we look at peptides, uh, its very tiny relative to like GLPs, which can be upwards of 50 amino acid depending on the one.

3:50 Like I said, it's a fragment of alpha melanocyte stimulating hormone. It is the C terminal tip of that molecule residues 11 to 13. So think of KPV as the active tip a much larger molecule, the part that carries the anti-inflammatory punch. What's really cool is we have this hormone called alpha-melanocyste stimulating hormones. This is where we to PT-141 and KPV. So all of those peptides are fragments of that total hormone. And what's pretty cool is we can isolate some of these fragments to have a specific effect.

4:20 That's what we'll see is that KPB, it doesn't cause you to tan or tanning, or it does cause to you have erections like PT 141 might, but it has an anti-inflammatory benefit. Basically we're harnessing anti inflammatory nature of the alpha melanocytes stimulating hormone and then applying it in a therapeutic application that we will cover today. This is pretty cool, the discovery story. So it goes back to 1989, James Lipton and Mary Hiltz at University of Texas Southwestern asked which part of alpha MSH carried the anti-inflammatory activity.

4:51 They isolated the C-terminal tripeptide and tested it in a mouse ear inflammation model. And they found that KPV alone was as potent as a high dose corticosteroid, which was a landmark result. Again, when we go back the therapeutic applications, How rampant is the use of corticosteroids today? I'm not saying that they shouldn't be used for the certain popular or certain cases that are popularly used. For when we look at KPV, we can get so much out of it that doesn't have the side effects of the corticoid steroids.

5:21 And 30 years later, that paper that found the field and we have preclinical research. Obviously, this was on the category two list. We'll talk a little bit about that today. But let's look from a system level what it looks like. So we have this thing called NF-kappa-B, which is a transcription factor. It travels into the cell nucleus and switches on inflammatory genes. Think of NF kappa B, you probably hear that a lot on different podcasts and everything, think about that as a master switch for inflammation. Basically, it's going into cell and saying, hey, genes, we are going to express towards inflammation, So in chronic disease,

5:55 the switch gets stuck on. And so whatever it is, whether it's autoimmune disease. Whether it was an injury, things like that. It's going into the cell, switching on this inflammation, and then it gets struck. So we're in this perpetual state of inflammation. We have inflammatory bowel disease rheumatoid arthritis, chronic tendinopathies, eczema, psoriasis. All of these have this NF kappa B transcription factor turned on, And what KPV does, especially that BPC and TB 500 don't directly do, is it blocks NF kappa B at the nuclear entry step.

6:27 The alarm tries to fire and gets shut off at a door. So think of KPB as blocking this NF Kappa-B from being able to get into the cell. And you see the little diagram there with the NF-kappa switch off, we have no inflammation. When the NF-KappaB switch is stuck on, We have all these inflammatory issues that so many people seem to struggle with in the world today. For whatever reason, we don't have to get into that, but obviously it's something that is rampant in our society today. Now, let's look at the mechanisms of KPV. So, KPB blocks NF-kappa-B at nucleus.

6:58 NF kappa B's active form, which is the P65 subunit, needs a transport protein called importin alpha 3 to enter the nucleus, What KPV does is it competitively binds to important alpha-3 at the exact site P65 uses. So KP-V gets there first, P-65 cannot get in, and inflammatory genes do not get switched on. So think of this as kind of saying, hey, no, you see the picture there. If you're watching the video, there's a door and it's locking this NF kappa B from getting in.

7:28 So this is unusual for a very small peptide. Like KPV, most anti-inflammatories work upstream, a la BPC and TB 500. KPB works at the transcription factor itself, which is a more precise and direct intervention. And again, to extrapolate that to practical use cases, this why I feel like it so powerful and so underutilized because people don't realize from an inflammation standpoint what it is doing to directly block this inflammation. Let's look at the mechanism two. We have PEPT1-mediated uptake. So KPV is a substrate for a transporter called Pept1, which normally pulls dietary peptides into intestinal cells.

8:06 In a healthy colon, PepT-1 expression is low. And in an inflamed colon particularly in someone with IBS or IBD, pept1 gets upregulated. the inflammation itself increases the transporter that pulls KPV into the cell, which means that KP V concentrates exactly where it's needed. Most drugs would kill for this kind of targeting. And then again, if you look at the steps there, we have a healthy colon, low KPB uptake, then the KP B dose is administered and you could do that orally or injected. Then we an inflamed colon to which we this pep T one expression.

8:39 The KP concentration, the disease site, at the site there, specifically in the case of the colon, which is pretty cool. Then we have mechany, or not really mechanism three, but what it does not do, it is not activate melanocortin receptors like most of other alpha MSH peptides. And despite coming from that, is does activate those receptors. We don't get any sort of skin darkening, KPV and melatonin to share a parent molecule but hit completely different targets. There's also no appetite suppression. there's another peptide I forgot to mention called Set Melanotide that is a fragment of alpha MSH that causes appetite suppression.

9:15 We don't get any appetite changes, obviously no libido shift, no flushing or anything like that. Again, a very clean mechanism with none of those negative side effects with the other alpha-MSH peptides. Now, why is it unique relative to some of the anti-inflammatory peptide? It's the only commonly used peptid directly blocks F-kappaB at the nucleus, not upstream. We also get smart tissue targeting. It concentrates an inflamed tissue through PEPT1 upregulation. Like we talked about, the disease state enhances delivery. Just as a side note, what's pretty cool.

9:45 I actually was reading a paper the other day. They've actually developed an oral version of KPV that is able to make it through the gut barrier to actually reach inflame tissue in other parts of the body. which is pretty cool because if you look at like BPC, even oral KPV, it's healing the gut, but we're not necessarily getting healing in our knees or our shoulders, wherever we might have an injury. But it does appear now, at least in rat studies, that we have a way to modify the delivery of KPB to take it orally but actually reach the target tissue. However, we can always inject it until that's available to use to reach to the inflamed tissue, for instance, if we ever hurt shoulder,

10:21 elbow, knee, something like that. We also have a very clean side effect profile. I will say KPV is one of the least side-effect peptides or least-side effect-inducing peptide that I have ever seen. Maybe once or twice I've heard of people saying that they have negative reaction to KPB in those cases. My guess is that that was a sourcing issue, not necessarily a KP-B related issue. But for people with MCAS or people that are very sensitive, you guys all know these people. You might be one of them. Some of the people I call the, no disrespect towards them, they're just the nothing works for me people, They have a reaction to almost everything.

10:55 And a lot of cases, I think that's very real. I'm not saying it's a psychological thing. It's real because their immune system is just basically being pushed to the brink. and I Think KPV is one the peptides that I would introduce to those people because it is so gentle. They're so small of a side effect profile and it has such profound healing. effects. And it's also one of the few peptides because of size that has real oral bioavailability, which is rare and obviously clinically valuable. Now I won't sit here and tell you that taking oral KPV is going to heal a torn meniscus or a torrent labrum.

11:25 However, it will help the gut, especially for these people that have oral issues, but I'm a fan. We'll talk about this a little bit later into the presentation. I am a big fan of doing both at the same time. A lot of people confuse KPV as something similar to BPC 157, but they're completely different. And that's why KPB I think works synergistically, and also is so unique. Honestly, if I were taking my Rathers, I would take KP over Bpc. Let's look at some of the people that do really well with KP. The first one is going to be the IBD patient. These are people who have Crohn's disease, ulcerative colitis, KPV has the strongest preclinical case here, so PEPT1 upregulation in inflamed colons means

12:02 the peptide concentrates exactly where the disease lived to be able to suppress that inflammation. Obviously, oral dosing makes the most mechanistic sense for this population. Again, I'm a fan of taking the oral and injectable at the same time. But again, it doesn't mean that it would be the only thing. There are other types of medications, BPC, lorazetide, other different peptides that also work really well to help heal a gut, but again KPV would one of the ones that I would lean on. We have leaky gut and IBS, people that really get maybe not as far as Crohn's disease, would have bloating, food sensitivities,

12:34 post-infectious Ibs, gut symptoms that flare with stress. I think especially for the people who have severe food allergies or food sensitivity to like gluten or dairy, KPV can be a game changer for them. It addresses the inflammatory component, which is often present, but not the whole picture. And it's the best, you obviously get best results when combined with diet adjustment, stress management, and then other peptides like BPC. Then too, I think one of the most underused cases that people fail to realize that works so well is for people that have plateaued when it comes to a healing

13:05 perspective on BTC and TB 500. Someone running BPC plus TB 500 over chronic tendon injury or stalled rehab often plateaus because inflammation is the rate limiting step. I think if I could drive home one point today about KPV is that when it comes to healing, inflammation, is, the, rate, limiting, step, and we look at Bpc and TB500, they rebuild tissue through nitric oxide growth factor and remodeling pathways, but they do not directly, indirectly, suppress inflammation. And so when we pair KPV with both of those, it breaks the plateau by addressing the inflammatory environment that prevents repair from sticking.

13:42 And again, think of KP, if you're someone that's taken BPC and taken TB 500, I'm not going to say KP will fix everything, but if he's really stalled out, maybe you got good progress with those. For whatever reason, you just could not get the injury fixed or the healing issue fixed. KPB will often be something that can help you break through that plateau. We look at another ideal candidate. I love KPV and the use of autoimmune skin issue in MCAS patients. And so for auto-immune flares, rheumatoid arthritis, psoriatic arthritis and auto immune skin conditions, all of those involve some sort of NF kappa B dysregulation,

14:16 which is obviously KP's primary target. You can apply it topically as well. Well, I haven't talked about that yet, but KPB topical in addition to the injection and oral works really well, chronic skin, conditions like eczema, Psoriasis, rosacea, Topical compounded cream plus oral or sub-QKPV for combined local and systemic effect works really well. And then lastly for MCAS, which is Mass Cell Activation Centrum, many practitioners report meaningful benefit and they like to start very low and titrate slowly, obviously, because that's usually a very sensitive population, but absolutely seem to do really in those people.

14:48 Should anyone skip it? Well, I think this is one of the ones, again, there's almost a use case for everyone, but if your main problem is not inflammation, it's not going to help with sleep apnea, thyroid dysfunction, hormone dysfunction and things like that. It's also not gonna give you a performance edge. So I wouldn't take KPV thinking that you're gonna train harder or anything like, that it could help you recover better for sure and have a better gut, which will then help obviously with performance, not directly. Again, if diet, sleep and stress are under address, KPV will not fix those. And it's not going to, you might get a little bit of benefit, but if your underlying foundational issues are not fixed, not gonna happen.

15:23 Obviously avoid during pregnancy, just cause we don't know. We don' have any sort of data around that. and then separate KPB from antibiotics, ACE inhibitors, or things like that, because those can also work on PEPT1 competition. So at this point, we just don''t know with some of those drugs that also worked on. Pepti one uptake. Let's look at dosing. And so we have the tier one dose. I would say the lowest dose, and this is going to be, if you're just introducing someone, maybe they're more sensitive, I was at 250 micrograms once daily.

15:54 You could do this oral and an oral capsule or subcutaneous. and I will do for four to six weeks. This is best for low grade chronic inflammation or general healing and longevity stacks. this wouldn't even be this would not be a bad idea. It would be good idea If you just want to have some background anti-inflammatory, not something that you necessarily need to heal. But sometimes I will just do this when I'm traveling, if I am exposed to non-organic food that I know I might have a predisposition to, have more sensitivity to. I love carrying KPV when i travel, just because in instances like that it really helps my digestive system if i'm exposed.

16:30 Or if you're traveling out of the country, maybe you are not used to the water, I was recently in Mexico speaking, at a conference and I always carry KPV with me and my gut seems to do really fine anytime I'm drinking water or eating food that I am not usually exposed to. Again, that low dose is enough to nudge inflammatory toward or downward without committing to a heavy protocol and a good starting point for people new to KP or if you just want something in the background. Tier two is going to be active healing and this is gonna be 500 micrograms once daily sub-Q or 500 mcg twice daily oral.

17:02 I would do this for at least six to eight weeks and it's gonna best for active gut healing, chronic injury, mid to moderate autoimmune and most skin conditions. And this I'd say is like the average dose of KPB. So this gonna if you're running a cycle where most people will lie. Tier three is going to be the severe inflammatory presentations. I would say 1000 micrograms per day. You could split this up into two doses, one in the morning and one of the evening. And in my case, when I've used this for injuries, it seems to work really well to take it once in a morning. For instance, I had unfortunately a really bad reaction to testosterone that I injected once upon a time.

17:38 And I had this huge swollen knot and I would take KPV in the morning. I injected KP V more near it and it would work great. But by the afternoon or the evening, the pain would start to come back and would inject KP again and kind of, kind almost act like an ibuprofen in a way to just help relieve some of the paint and suppress some inflammation. And for anyone that's ever just gotten a bad reaction to injections, you know how painful that can be when you have a lump and everything. And so I love KPV for that case, but that would say where I would be where you would have this more severe inflammatory presentation.

18:09 You could go up to one milligram per day. I've gone higher than one milligrams per. Day. Honestly, the difference between two and three milligrams versus one kilogram doesn't seem to have that much more of an effect. Meaning that I think one milligram for a lot of people, I'm not saying that you can't do it. You can obviously do whatever you want. I Think beyond one milligrams per day, it's probably just unnecessary. Meaning, that, you could only block NF kappa B and have so much of an anti-inflammatory effect. It's kind of like a completely different analogy. But if we look at something like ibuprofen, 800 milligrams versus 1600 milligrams of ibuprofen, how much is that really going to make a difference?

18:42 I don't think that much. Usually 800 mg is kind of the big dose to which someone will really say like, okay, I'm feeling it and I need to go beyond that. I think it's very similar in principle to KPV. Beyond 1 mg, in my own experience and other people that I've worked with, that's much more benefit of going above and beyond 1 milligram per day. And again, even if you're new to it, you could start low and then go up to here, but I think that's great. And you can obviously do this in oral as well. So you combine another 1000 micrograms orally per day to help alongside that.

19:13 Again, best for IBD flares, severe MCAS and post-surgical inflammation. For this level, I would say two to four weeks, and you go back down to the 500 micro grams per range. When we look at dosing by purpose, let's talk about gut healing first. The oral KPV dose is 500 micrograms twice daily for six to eight weeks, pairs very well with oral BPC at 500, micro grams twice, daily as well. This would be for ulcerative colitis, Crohn's, IBS, leaky gut, food sensitivities, and post antibiotic gut damage. I will take an aside briefly and just say as someone that has struggled with post-antibiotic gut damaged when I was younger,

19:49 KPV could be a miracle cure. I just wish when I was between the ages of like 17 to 21 that I had KPB because I on heavy antibiotics as a teenager for my acne and that ruined my gut. And I have all of these gut issues for at least six to seven years after taking that as teenager. For someone that does have to use antibiotics for whatever reason, I love KPV. Now, I wish I would have had KP in too, because it would've helped with my acne. So I wouldn't have to be on antibiotics in the first place.

20:20 I could have just used KP and that probably would of helped a lot with the acne, even when I have acne flare ups as an adult here and there, KP works really well for that. And then we also look at the mechanism when we combine it with BPC. Bpc drives endothelial repair and Kpv calms inflammation that prevents repair from sticking. And that's why they do really well together. For anti-inflammatory and injury, I like subcutaneous KPV at 500 micrograms daily for four to eight weeks, then two to four weeks off. Depending on the inflammation, usually that's enough time to really cover a lot of suppressing the information.

20:55 But if you need to do it longer, there's nothing wrong with that. It's just one of those things we tend to better because of the way peptides work. When we cycle them, we're more responsive to them. When we look at an injury stack, I like subcutaneous KPV 500 micrograms daily layered onto standard BPC plus TB 500 injury. And you could run this for six to 12 weeks. I think it's highly underrated for chronic tendinopathies, installed rehab. Also too, this is one of those ones, just use discretion when it comes to cycling, because I would rather someone use BCC, TB500 and KPB altogether until the injury is healed rather than cycling off just because they've been on it for eight weeks,

21:29 I would say, hey, use that until you're healed, especially if things are moving in the right direction, rather than just cycling off at some arbitrary number. We look at Skin and MCAS. I really like compounded cream at 0.1 to 0,25%. There are research companies out there, which I will not name in a public video, but that now have KPV cream, Which is really cool because two years ago I was just thinking, man, it would be so cool. Cause there's, there is clinical literature around compound to KPB cream. But there was no one selling it. And I'm like, Man, It'd be cool if you could get KP into a cream and actually commercialize it and now thankfully to the work of a lot of really smart

22:03 and really good people, we do have that. And you could also do this as a microneedle before topical application increases penetration significantly. And then what I would also is combine it with oral and or subcutaneous for a systemic effect, but it does work really well for skin. Anytime I might have like a pimple break out here or there, I'll rub some KPV on it and it really does well to bring down that inflammation. For the MCAS protocol, I start very low. Maybe even for some of those people, you could start at 250 micrograms once daily oral just to help heal the gut environment and then watch for reactivity.

22:34 Some patients tolerate doses up to 500 micro gram twice daily after titration, especially for those MCAs people. And then you can bring in the injection after. So again, if there's people that are very sensitive, maybe just start them on the oral and make sure they do okay after a couple of weeks and introduce the injectable as well. Timeline, what should we expect and when? I say in week one to two, most people are going to see gut symptoms improve on oral protocols and people that if they do have any sort of reaction, they would usually settle there, although it's very rare.

23:05 Weeks two to three, injury benefits appear as reduced morning stiffness and faster recovery between sessions. Weeks three to four systemic inflammatory effects produce objective change, usually energy and well-being shift, especially if it's someone that has had systemic chronic inflammation via rheumatoid arthritis or Crohn's or something like that. And then week six to eight is typically when we see an inflammatory lab markers like HSCRP move in the right direction. Skin conditions usually begin to respond, although they can be highly variable, but KPV, I will say this, just to set the expectation,

23:38 is not a peptide you feel on day one, usually is going to be a cumulative effect. Now, that doesn't mean if you have an acute flare up that you won't get benefits on Day 1, but for someone that is dealing with that chronically over a long period of time, it's not going happen overnight. And so you got to give KPB a chance to work and usually that's going take at least a six to eight week window to do so. Now, why should we cycle KPV? It's a very short peptide. It does not have a classic desensitization problem, and there's no receptor that can technically desentitize it. However, we cycled for these reasons.

24:09 One, it's always just out of safety precaution just because of the peptides. We don't have lot of long-term data. It also gives us an assessment window that breaks down to see what KPV is actually doing. And so if symptoms return, you know the protocol was working and that you could go back on it. Then also we get cumulative exposure. It reduces the cumulative of exposure as a conservative precaution until we have longer-term data. I think a lot of times with these peptides, it's just something, do I worry about that with KPB having issues long- term? Absolutely not. But we just don't know what we don t know.

24:39 We talk about peptide we're always going to be a little bit more sensitive to it when we cycle on and cycle off, and then we also get the benefit of just having that to where we don't know what we do not know, so it translates into making sure that we can monitor ourselves as we go along. Most active protocols cycle for six to eight weeks on and two to four weeks off. This is just kind of standard. Autoimmune patients, I would say eight week on, four week off, these people are oftentimes going to need those longer cycles. And then you could use long-term maintenance of continuous low dose at 250 micrograms daily for chronic conditions with periodic breaks every few months.

25:12 I think it's totally fine. And then if you're doing the higher dose of acute flares, I like targeted short cycles of two to three weeks for acute flairs, then you can step down for maybe a week or two, to go back on to the high dose. Again, it's simple, but just use your discretion based on the benefits that you need or the benefit you are expecting to get. Let's look at some off cycle expectations. Benefits often persist for several weeks off-cycle because again of the cascade that's happening in the body. And this is a sign the protocol shifted the tissue meaningly. But again, if you cycle off and some of the benefits seem to dwindle, you probably needed to stay on for a longer period of time.

25:45 If symptoms return quickly, the underlying drivers are still present. Again, consider longer cycles and work on things like diet, sleep, and stress. And again if go eat, let's say, a bunch of fried food and you're expecting KPV to stop the inflammation of your fried foods, it's probably going to help with that. However, I would recommend that you don't eat fried-food three times a day every day. because no matter how much KPV you have, you are still overloading the body with inflammation. Kind of the same thing when it comes to injury stuff. I talk to people all the time and I'm like this too, because I come from an athlete's background, but I've talked to People, maybe they have tennis elbow, their knees hurt from playing basketball or something like that.

26:17 And honestly, if you're using something repetitively that is inducing more and more inflammation, no amount of KP in the world is necessarily going to stop the onslaught of inflammation It can help heal it if you take time off. But again, if your not being intelligent about how you do things, whether it's on the diet side or the lifestyle side, KPV is not going to fix all those things. It could definitely help, but it isnt going fix it. If the benefit holds for weeks after stopping the protocol shift of the tissue meaningly, and that is the assessment value of cycling, it tells you whether KPB was doing real work or whether the underlying condition needed more foundational attention.

26:50 And the sustained off-cycle benefit is best signal that KP is working as intended and hopefully healed the environment Going forward, let's talk about sequencing. So if you have a house on fire, you do not start hanging new drywall while the flames are still active. KPV calms the inflammatory environment. PPC, TB 500 and other regenerative peptides do the rebuilding. Both jobs need to get done and the order matters when inflammation is severe. What I like to do first, especially for someone with gut issues, is to use KPV 500 micrograms per day to calm inflammation and then in weeks two,

27:22 that would be week one, in week two we could add BPC and continue with the KPB and in phase three we would take a break or we'd go to a little bit more of an aggressive protocol depending on how the person is doing. But what we want to do with KPV is call the inflammatory environment before introducing the rebuilder. Whether that's BPC, whether it's TB 500. And so starting BTC in a severely inflamed gut fights against the backdrop rather than working with it. It's not that it doesn't work, but it just works so much better with KPV. For injury, I like kind of the same thing.

27:52 So 500 micrograms a day sub-Q to reduce inflammation for the first week. Then we can add in Bpc and TB500. to help with that because now we're calming the inflammatory environment. And then by 12 weeks, we can say, okay, is this something that I need to continue doing? Or is it something I can cycle off because I'm pretty much healed? And a lot of times you can come off of the KPV because you've pretty And then same thing when we start BPC and TB amid high inflammation, it's not always going to work well. And I think that's something now that we have so many more people using peptides that I'm seeing a lot, is that just people are using Bpc and Tb500 thinking

28:28 they're going fix everything. A lot of times it is not doing enough to suppress the inflammation. Looking at reconstitution, very simple. Obviously, KPV comes to lyophilized freeze-dried powder. You have to add back water. Most vials come in five to 10 milligrams. If we have a five milligram vial, I would say put two mLs of water, if you have 10 milligram, vile I'd say, put 2 m Ls water and again, just know the math. if have you a 5 milligram Vial that you add two milliliters of Water, it's now 2.5 milligrams per milliliter. And so if wanted a 500 microgram dose, that would be 20 units.

28:58 Very simple, you can see there on the slides if want to screenshot that. I know some of you guys are like, oh, do you have to talk about this? But it's something that I get asked all the time. Same thing. If I have a 10 milligram vial, add two mls of water. Now five milligrams per milliliter. So if I wanted 500 micrograms, that would be 10 units. And so there you go. There you. Have it with the dosing. Again, very simple with a reconstitution procedure. I've got this on the slides, but you know what to do. Just make sure you're shooting the water down the side of the bottle. Let it settle. Kind of don't shake the bottom. You don' need to shake it or anything like that. It'll mix.

29:28 and then injection wise 29 to 31 gauge insulin syringe 5 16th those are the ones that I use 31 gage 516 through the easiest sub cue into the fatty layer just under the skin I would inject this if you have a site injury next to the site or as close as possible without irritating it or if You were injecting it for systemic effects you could just inject into The belly fat and that's usually Good enough. I like to do down at a 45 degree angle, wherever I'm injecting. For storage and travel, obviously same thing, very simple with peptides.

29:58 You know, the rule of thumb is used within 30 days, but I've used KPV that is six months old. And so that's what the science is always going to tell you. But it's just because we don't have a lot of science to validate that they do last for longer. Sure. Maybe there is some less potency the longer it sits, And so traveling wise, just obviously keep it cold with cold packs in your insulin cooler. What to track? Let's look at some subjective markers, basically just how you feel. Obviously look energy and sleep, energy level and quality rated one to 10 each morning.

30:31 Stiffness and pain, that's going to be the biggest one, especially if you have arthritis or inflammatory issues in you joints. This could be wonderful for you, but morning stiffness is a sensitive early signal. Then gut symptoms, obviously that's huge bloating, pain, post-medical energy, stool quality, skin and mood, and also it's just track your skin quality because that will really help as well. Let's look at some of the objective biomarkers. The biggest one is HSCRP. This is the most sensitive systemic inflammation marker. And this is what I would primarily watch for people on KPV.

31:01 A lot of people might not even realize they have a high HS CRP to which I've seen KPB bring that down from like a five down onto a one, which works really well, Ferritin can be a chronic inflammation marker. Elevated ferritine often tracks with inflammatory burden, and so you would want to look at that if you're using KPV on blood work. CBC with differential, which is complete blood count, tracks immune cell populations and general health statics. Obviously comprehensive metabolic panel. You won't really see a lot change unless it's an inflammatory thing. And then again, just condition specific. So there are any specific things like antibodies or things that KPB can work really well for those.

31:35 Stacking, let's talk about this because I love stacking with BPC-157. I would say KPV and Bpc-147 for inflammation is perhaps the highest value pairing in clinical practice outside of, again, using a GLP. Bbc rebuilds the tissue through nitric oxide and growth factor pathways. The anti-inflammatory effects are secondary. And then we have KPB, which provides the primary antiinflammatories layer that BPc lacks. It also directly blocks NF-kappa B. And again, dosing wise, I like these in a one-to-one ratio, so you can use 250 to 500 micrograms of each, one to two times daily for six to eight weeks.

32:08 Again, that would be great for IBD, leaky gut, IVS, post-antibiotic damage and chronic tendinopathy and post surgical recovery. Then we can layer in TB 500 as well. That's going to add in a remodeling and angiogenesis effect. You could do the TB, BPC, and KPV all on a one-to-one ratio, or you could higher doses of TB500. It just kind of depends on what you want to do. So use discretion there. If you're getting a blend of them, for instance, in the clove blend, which would also have GHK, obviously they're usually on one to one ratio there,

32:39 Some other peptides that people don't realize that pair really well with KPV, and we'll talk about them. One is lorazetide. Lorazotide is an oral peptide, it is so underrated too. And it was originally developed for celiac disease, but the benefits go beyond it. Even just doing celiax disease. Lorzatide tightens tight junctions while KPB addresses the inflammation driving the leak and their complementary mechanisms that target the same problem from different angles. KPV and LL37 are amazing together for gut pathogen overgrowth. KPB counterbalances Ll37's inflammatory tendency, which allows the antimicrobial benefits without the inflammatory cost.

33:15 Not that that really happens if you're doing L 37 at a low dose, but for people that have SIBO or any sort of bacterial issues or viral issues, or even mold issues. Those two together work amazing because you get the reduction in inflammation from the KP and then the direct anti-inflammatory microbial benefit from the LL37. Then we have KPB and thymus and alpha 1 for autoimmune conditions. We get the TH1 immune balancing plus the inflammation suppression. Again, very two complementary peptides are very synergistic together and I love using those together as well.

33:48 And then let's talk about TRT. There's no interaction and KPV does not affect hormone signaling. So you can stack it freely with TRD. Although I think it just works well for people that are on TR T and they still have inflammatory issues. Cause that happens. And in KPB and GLP one agonist, we have no pharmacological interaction with your Zap Tide, SEMA or Retatrutide. It may help mitigate the inflammatory component of G GL P one GI side effects. You can sack freely. I really like for obviously using GLPs to suppress inflammation, but for do have those GI Side effects, We actually just did a call in the private group. about how to manage GI side effects on GLPs.

34:20 And I love using KPV to help some of those issues that people have just because it kind of reduces the flare up that some people in their gut. So you can use those definitely together and they work really well to suppress inflammation. Let's just talk about some supplement and nutraceutical support. I really love glutamine. That's something I don't talk enough about.

34:55 We have PEA, which is a mast cell stabilizer. 300 to 600 milligrams per day of PEAs works really well. I love that one. We also have zinc and carnosine 75 to 150 milligrams a day for gastric mucosa support as well studied for upper GI barrier integrity. Then we have N-acetylglucosamine 500 to 1000 milligrams For gut barrier substrate, it also provides structural building blocks for the mucosal layer. And so I really love if someone has gut issues pairing these things with KPV.

35:26 Let's get into some troubleshooting. So what if you say, I don't feel anything. One, the dose could be too low. Sometimes maybe that 250 microgram dose is too, low and you need to go up to the 500 micro grams to a thousand micro gram range. You can definitely do that again. Beyond a thousand, I just don't see that much more added benefit. The durations could be too short. I mentioned that earlier. You just want to make sure you have a cumulative exposure of at least six to eight weeks to really assess whether it's working or not. Could be the wrong route. Maybe you're just taking the oral version because you were scared of injections. That oral and the sub here are going to work synergistically together.

35:57 Obviously you could use either one, but I like using them both together, obviously just verify source quality and then Again, it might be the wrong peptide. If inflammation is not the underlying issue, It's not really going to do anything. And again, maybe you have a torn shoulder and use KPV and it reduces inflammation, but it's also still not going help build the structural area that BPC and TP500 would, which is why they work so well together. Injection site reactions would be probably the most common, But again it is very rare in this case. So just make sure that you're rotating injection sites. There is the possibility that you could get a die-off reaction, especially with oral KPV and dysbiosis people.

36:32 Just reduce the dose by half for a week, hydrate well and consider binders like charcoal or zeolite. Mild GI discomfort can come with the oral dosing. Again, it's a lot of times it could be cleaning out whatever's in the stomach that could that could be causing issues there, and you could just take the injection that might not have as bad of an effect. And then if you get redness, fever, warmth, or pus, it indicates a possible infection to just stop immediately. Again, KPV would be good to benefit infection, but if that were to happen, just to stop it immediately, When to stop if you get severe or spreading injection

37:04 site reactions, persistent GI symptoms beyond two weeks, neurological symptoms, worsening autoimmune condition, or any anaphylactic type reaction. Again, these are extremely rare. I would say the worst probably or the most common would be the worsing auto immune condition just for whatever reason. Some people are just so sensitive to anything, but I will say relative to other peptides, I always put this in there just because it's possible, because anything is possible with peptide and I've seen it all or feel like I see it at all at this point. So just be aware of that. But again, very rare in the case. case of KPV. Let's talk about the legality for a second.

37:35 In September 2023, it was placed on the category two list, which blocked compounding pharmacies from making it, unfortunately. The FDA proposed removal of KPV from category 2 in April of this year, along with 11 other peptides. July, June is the time of filling us with July 23rd and 24th, the PCAC will review KPB, BPC, TB 500, MOTC, DCIP, Epitalin, and CMAX. to remove from the 503A bulks list, which means that they will not be FDA approved, but it will now be okay for compounding pharmacies to make them a lot easier for doctors to prescribe them.

38:06 And again, that's kind of our best hope right now with KPV. I doubt it'll ever become a pharmaceutical drug. Just some FAQ, again, BPC versus KPV. Bpc is 15 amino acids, rebuilds tissue through nitric oxide and growth factor pathways. KPv is three amino acid directly blocks NFKB, which is the master inflammatory transcription factor and the anti-inflammatory effects are primary rather than secondary. And again they work really well together. When we look at oral versus sub-q, the oral is the best for gut-focused work.

38:36 We get the PEPT-1 upregulation in inflamed gut tissue, which means the KPV concentrates exactly where we need it, and the disease state enhances the delivery of the KPV. Also, subq is better for systemic effects, so for autoimmune, tendon injuries, skin, MCAS, it bypasses the PEPT1 competition with other medications in the gut. And again, you can use both of those together if you really want to do it. How long will I feel it? One to two weeks, usually obviously gut symptoms improve. Two to four weeks usually systemic effects produce subjective change.

39:06 Then six to eight weeks is typically where we'll end up seeing the labs catch up with the practical effects. And again, just one of those things may take time. Again, with skin conditions, those can be highly variable. In some cases, it may two weeks. Some cases it might take up to several months. KPV is not a peptide you feel on day one. Give a fair trial of at least six to eight weeks before drawing conclusions if it works for you. Pregnancy, we just don't know, so I would avoid it. Pediatric use, this is a thing I get asked a lot, especially about kids with inflammation or acne issues, things of that nature.

39:37 There's no data in children. And again, we don't really have anything to say. I personally would feel safe in my own children using it, but again you got to make the decision for yourself and that's not something I'm going to publicly go recommend to people. Then again when we look at long-term continuous use, We don' have any human data beyond a few months. Periodic breaks recommended every six to 12 months at least at a minimum as a conservative precaution. i would just not do it every day again. Not that I am worried about it but we just don''t know what we dont know. People want to ask a lot of times because of the nature of structure, will it cause skin darkening because it's an alpha melanocytes stimulating hormone peptide?

40:11 No, it does not activate the melanochortin receptors that drive pigmentation. And again, when we look at full alpha MSH or melanotan-2, those do activate melanokortine receptors, but KPV does no activate those. There's no skin-darkening, there's not appetite changes, There is no libido shift and there is not flushing. If you do get those side effects, you probably don't really have KPB if that is because I've used a lotta KP and I have never had even remotely any of those What about long COVID and MCAS? It's mechanically plausible, but not proven in trials. Practitioner reports describe meaningful improvement in some patients, particularly those with gut symptoms or persistent fatigue tied to inflammation.

40:45 MCAs anecdotally yes, and tons of people that I've seen. The parent alpha MSH has mast cell stabilizing effects that KPV partially retains. I would start very low and watch for reactivity and slow titration matters more than hitting a target dose quickly in this population. And you can always start lower with those people just because they oftentimes are more sensitive, cost of sourcing usually going to be somewhere in the, you know, 50 ish to a hundred, depending on the vial size. If you're getting at research from the oral side, usually a 100 to 200 bucks per month, because the orals tend to, be a little bit more expensive.

41:17 You can obviously use it in a clove blend and save money. That's why those blends are so popular. And yes, they do work together. I'm not someone that tells you you can't inject. those specific peptides together. I've done it. They all seem to work for me. And we also have lab data now to back that up that they're all stable even after 30 days of being mixed. Again, just know what you're doing when you are looking at quality indicators. Why isn't KPV more popular? One, it's an economics problem. Obviously, its cheap to synthesize and weak patent protection, so it is not going to be something that gets pharmaceutically introduced or even from a

41:47 supplement standpoint. There's not gonna be a lot of money made off of it. No pharma company has funded the larger clinical trials that would generate brand recognition and there's no financial incentive to do so. And obviously, two, there is an awareness problem, the peptide community defaults to BPC and TB 500 because those are better known. They have more of a track record. They're better known, they're just in the common consciousness that people know them. It's not because they are more effective for inflammatory conditions. The result is a peptide that works well but lives mostly in clinical practice rather than established headlines. We have that PCAC review. Hopefully that's a favorable outcome and hopefully that will actually drive many more doctors to prescribing KPV and many people to using KPB.

42:20 I'm hopeful that that go well, but you never know when it comes to government. Let's talk about some next generation compounds as we get close to the end. We have this thing called KDPT, which is a closely related analog with a longer half-life. I think this could be really cool. There was a 2011 study on colitis. It's still in research, it's not commercial. Promising, but this may or may never be brought to market. And again, I thing it is cool, again we don't know. You have thing this called CKPV-2-dimer, stabilized version with anti-fungal activity, maybe a little bit of benefits of LL-37 without having to take that.

42:53 Early clinical interest, but development has been quiet in the structural stability advantage over the monomer. Now we have nanoparticle delivery. This is what I was kind of talking about earlier. There was a lab that developed TEPT1 targeted nanопarticles for IBD and the KPV plus this thing called FK506 paper is the technical frontier. You're from approval, But it could be really cool with some of these alternative delivery of taking an oral version of KPB but also getting the systemic benefit of it. To sum up, KPV is the peptide that calms the room so the rest of the work can happen.

43:24 Inflammation is almost always the rate limiting step in most chronic conditions. KPB addresses that inflammation directly at the level of transcription factor that controls inflammatory gene expression. Again, it's not super flashy. It's a peptid you feel acutely, but it changes the nature of body and it helps with infrastructure. And again, we have 30 years of strong preclinical data across at least eight inflammatory models. We don't have completed phase two or phase three trials in humans. We probably never will get them, but the clinical use rest on the mechanism, preclinical extrapolation and decades of alpha MSH human safety and uncontrolled

44:00 practitioner observation and us in the research world, obviously that you guys are out there helping move the science forward by using it. There is a ton of anecdotal data that shows this works very, very strong. And then lastly, just use a well. Just remember with KPV, it's a tool. If diet, sleep, and stress are unmanaged, KPB is not going to perform to the level that you heard me talk about today. Those fundamentals are in place. However, KPV often does exactly what the mechanism will predict it does. It releases the inflammatory break and lets the healing happen. And if you ever run a peptide protocol that worked for a while and then plateaued, PPV can oftentimes be the addition most likely to break that plateau.

44:35 But just remember, it's a tool. It's not a panacea. it is not going to fix everything, but it can help you get going in the right direction if you're doing the rest of the work. And that is it for the slides. That is the masterclass on KPV. Hopefully that was informative to you guys. Again, I loved preparing this, filming this. I just loved this whole presentation. The process that went into it, because I'm so passionate about using KPB, is again one of peptides that to me I personally rank even over BPC. And TB 500 in terms of the effectiveness and what it does. Again, I said it before, but if I could choose KPV over those two, i would choose kpv every time because of The amazing anti-inflammatory benefits it has.

45:14 And I will say practically speaking, it works faster and better for me. to help heal any time I've experienced inflammation. And again, it's just one of those because of what it does. It's so versatile in the nature where some of these other peptides are extremely valuable for what they do or what the treat, but they just don't have the versatility of KPV. So I look forward to hearing you guys' feedback. Let me know in comments or messages that you send me what you thought of this one. I seem to be getting really good feedback on doing these master classes and obviously I love them.

45:45 to sit down and do long form videos like this. It's literally what I get excited is what i get out of bed in the morning to do. And hopefully you guys enjoy watching and consuming these as much as I enjoy doing them, especially in an age of social media and AI where everything has gone to short form and kind of just like try to have the goldfish attention span. I want to do the opposite. So thank you guys so much. Just in closing, I am so, so grateful for the support I get from you, guys, whether you use my code at places, you're on the email list or my private group. You share this with your friends and family, or even if you never do any of those things, but you just help support through viewing the content.

46:19 Whatever support you give me goes so far in helping me bring these messages to you. So just know that you are appreciated and your love. I will say that in every single video because it's important to me that, you know, that. And so I know it gets old for you guys that listen all the time, but thank you, guys, so much. It goes, So far and helping be whatever support. You provide to bring this message to, and I plan on doing it for a very, very long time to come.