NAD Benefits My Ultimate Stacking Guide for Energy Performance

0:09 Hey everybody, this is Hunter Williams. I hope you're doing amazing wherever you are in the world. Today's video is going to be my overview of the NAD plus cycle. Now I will preface this by saying I am by no means an expert on N80 plus. Um, so this video's going be more or less my interpretation and best communication to the layman on what N A D is in body and how this whole cycle works. So I was recently inspired, uh, to do this, um, from listening to Dr. Sandra Kaufman, talk at our live seminar in Tampa recently.

0:42 And everyone should go check out her work. She's one of the foremost experts on anti-aging and longevity medicine in the world that I know of, way smarter than me. But she did a presentation on exosomes and NAD+. And I was fascinated by the N, N A D plus conversation. Cause it's something, you know, like I've used N. A. D. I have used some of these other supplements to help support N and D, but they really have like a conceptual understanding. So, uh, after the seminar, I just went back. I was doing a lot of my own reading and wanted to put together something to kind of give you a framework for understanding NAD,

1:15 why we even would address NID and then also to what you can do from a very simple supplementation standpoint to optimize pathways depending on your goals. in my style and fashion. This is my best foot forward on understanding NAD and then communicating to you guys and how to use injectable NADA, 5-amino, 1MQ, and NMN and NR. So that's going to be kind of what we're going over today. If you want to learn from the best, go check out Dr. Sandra Kaufman. There's another guy named Dr G. that is also, I think, probably the most well-researched person in the world on NAD+.

1:48 So those guys are way smarter than me. But this is just my interpretation and teaching on it based on everything I've known in past and then just learning more from, especially Dr. Sandra. So as always, check out the Peptide Cheat Sheet. I do have Nad Plus on the peptide cheat sheet. If you are interested in knowing how to mix it, you can check that out. And obviously too, if you like conversations like this, we have the private community, fully optimized health, so you can head over there and check out because there's 600 amazing members that are geeking out on this stuff each and every day, hour by hour.

2:18 So without further ado, I'm going to share my screen. And today we're going get into the NAD plus cycle. All right. I am Hunter Williams and today's video is the NAD plus cycle explained and what to do to optimize it. So let's get in. Uh, it's going to be pretty thorough cause I wanted to make sure I did a good job of explaining this, but also explain it in a way that makes sense. And, um, hopefully it leads you to, uh, your own research as we like to say. What is N A D? So it stands for nicotinamide adenide, adonine dinucleotide.

2:49 N 80 plus. Do any of us have the disservice of having to pronounce that? So I'm just going to call it NAD+. It's a vital coenzyme found in every cell central to how our bodies produce energy and maintain healthy function. Now, where NAD really comes in is as we age, our levels drop off dramatically as do other things and, you know, that we use peptides and hormones to optimize, but they decline significantly with age and more particularly the decline with stress. So by age 50, N80 plus concentrations in most people have dropped around 50%, which obviously leads to fatigue, slower recovery,

3:24 metabolic inefficiency, we store fat more easy and cognitive dulling as get older. Now, when we look at the importance, so NAD plus toggles between the oxidant form of N80 plus and reduced form with his N A D H as it shuttles electrons and metal block reactions, which enables glycolysis, the Krebs cycle and oxidative phosphorylation to generate ATP. Basically, that's a long way of saying is N 80 plus is very crucial in energy production and regulation within the body. So we looked at restoring it, um, what is what this video is all about.

3:55 Um, we use supplementation through different pathways. to supplement and improve NAD+. We're going to have better muscle function, endurance, stamina, and even longevity markers, which obviously helps us stay long or stay youthful for the long term. So that's the whole reason we're doing this. Basically, Cliff Notes version is N80 plus is crucial to our energy levels as we age. And also too, when we look at like peptides and hormone stuff, it kind of is like not necessarily like I wouldn't say like completely independent of those things,

4:27 but it more or less is a different pathway that I think in getting caught up in the hormone and the peptide game, sometimes we kind of leave to the wayside. So let's bring some attention to it today. Let's look at NAD and cellular energy production. Like I talked about, NAd plus functions as a electron carrier cycling between Nad plus and Nadh to enable Oxidation which is just the release of energy and reduction, which has energy storage and metabolism. So at the heart of cellular any production is this whole idea of the NAD cycle.

4:59 Think of this is kind of a loop that's running in the body and we're going to get into like what's going on there. During nutrient breakdown, so when we intake food and then break that down for nutrients, NED plus accepts electrons and hydrogen which converts then to NH which it's high energy form. the mitochondria's electron transport chain to donate these electrons, driving the production of ATP and regenerating NAD+. So this continuous N80 plus to N A D H cycling is what allows glycolysis and the citric acid cycle to keep turning, yielding the energy that powers muscle contraction,

5:36 brain signaling, and all biological work. So again, kind of this loop that is running the body constantly behind the scenes. If N 80 plus isn't available to pick up electrons energy production stalls. So think of an 80 plus as the limiting spark plug for your engine. So we need this kind of as a critical component to be able to complete this whole energy cycle in the body. And when we don't have it, it kind is like pulling a sparkplug out of your car. Most people don' t have spark plugs in their engine, don t know too much about cars.

6:08 But I do not change my will. My dad would have failed as a father in his eyes if he wouldn't have taught me how to change, my own do other things in my car. But in addition to its redox role, N80 plus directly impacts cell efficiency and longevity via these things called sirtuins, which are a family of N 80 plus dependent enzymes. You'd probably hear srtuins. on a lot of biohacking podcasts and stuff like that. So what are they? Serturins are things like SERT1. They act as metabolic regulators that adjust gene expression to enhance mitochondrial function and stress resistance, but they can only work when sufficient

6:41 NAD plus is present. So they're helping with our metabolism, they are helping our mitochondrial function, but they need NAD plus to be able to work properly. So low N80 plus levels effectively turn the sirtuins off which impairs our cells ability to adapt and recover to stress and have energy. And then the NAD plus cycle also ties into pathways like glycolysis where NED is needed to sustain glucose breakdown. When we intake sugar to be able to break it down and fatty acid oxidation, which generates NAH. So in short, when we optimize any D plus levels, we're going to optimize the rate and efficiency of ATP production in the body across virtually every energy

7:16 pathway that we have. And so for a biohacker, fitness enthusiast, health optimization expert, uh, you know, that be all fancy ourselves to B this translate into more horsepower from ourselves. greater endurance, strength, and mental clarity, stemming from well-fueled metabolically youthful cells. So kind of think of NAD is a crucial spark plug in our car that is helping drive everything going on. If you pull a sparkplug, maybe or maybe the car doesn't work, but it's not going to work as well. For all intents and purposes, it is long way, complicated way of saying we need N.A.D.

7:47 for energy, which leads to muscle function, muscle building, fat loss, all those things. Um, now we have all these different things, right? So everyone gets confused because we've got NMN, we got NR, NAD+, IV, and NADA+, subcutaneous, intramuscular. How does this come together? There's a ton of different strategies to boost N and D plus levels, each with its own advantages. Today I'm going to build a framework around The simplest way that I use, in my opinion, that, I think, everybody could benefit from.

8:18 So the main approach includes direct NAD plus injections or infusions and supplementing precursors that the body converts N-A-D plus. Um, so injecting N80 plus is good, but that's just one piece of the equation. And I'm going to break down four popular options, which are the injectable NED, NMN, NR, and then 5-amino, Which is less understood in this cycle. People know about 5 amino and they use it like as a supplement. but they understand it less in this context. And then also too, because of the nature of being a research chemical, you hear way fewer of these biohackers out there talking about it as opposed to

8:54 NMN and NR, which is obviously very, very popular to hear in common bio-hacking parlance these days. So let's look at injectable NAD+. First, I'm a fan of this. I think sometimes it can be overblown, but I still think it's important. So injectable NAD plus delivers the coenzyme directly into the bloodstream via IV or IM shots, bypassing the digestive system for near instant bioavailability. So this is one that we have to inject. If we're injecting actual N80 plus, we to have inject it.

9:24 There are, you know, supplements out there that will say that you can take oral versions of them to my knowledge and based on my experience, I don't think any of the work. The precursors, yes, but not the N 80 itself. By injecting, we get 100% absorption. You know, it can actually flood cells with NAD and rapidly replenish depleted source. So this is often why people feel really good after they get a shot because you just get an immediate boost of mental clarity, energy, and mood with any D plus infusions or injections. People will often get it from a clinic when they're getting the IV to combat chronic fatigue and brain fog.

9:57 And then for high-performance professionals out there, injectable NAD is used for rapid recovery post-competition or during intense training blocks to accelerate cellular repair. I think I wish I understood this more when I was a competitive athlete because my repair would have been a lot better. My recovery would've been especially like after games or competitions, whatever. So, you know, it's also been explored when we're doing talking about the injections for cognitive enhancement and neurodegenerative conditions,

10:27 and even as an addiction, addiction detox aid, which I've had heard good things about. And I think to one of the reasons people get An outsized benefit from injectable NAD, whether it's the IV or the IM, is because a lot of people drink, and when you drink you rapidly deplete N.A.D. levels. And so when your drink then you can do an N-A-D injection and you don't feel as bad or as hungover. So high dose Nad may also help reset brain chemistry, again because of this energy cycle that's going on that we explained. that oftentimes is impaired from alcohol, stress, lifestyle, poor sleep, and all those things.

11:00 So what are the downsides? One, IV NAD is just tough. You obviously need medical supervision and it's a higher cost. than just buying it as a injectable lyophilized powder that you then may or may not reconstitute on your own and use on yourself, um, intramuscularly, but, uh, NAD IV therapy can cost hundreds of dollars per infusion, depending on where you live, being on how much clinics charge around you. Um, and it only lasts really a few hours since they need plus must be for, drip slowly. So a lot of times when you do these IV infusions, you're sitting there for like three or four hours.

11:33 And if you to do the push, I've never done this. I'm just speaking from people that I talk to, it can be quite painful if accelerate instead of just getting the drip done. Now, what I have done quite extensively is the intramuscular injections. They deliver smaller amounts typically. The IV and AD spikes levels dramatically. The effects are acute. Any plus has a half life in tissues. So without ongoing precursor supplementation levels may taper in days. Um, so it's kind of think of it as like the fast and furious approach is great for immediate performance needs or deep, um,

12:05 recovery sessions, but not the most practical for daily maintenance. Now I think the IV yes, less sustainable cause you got the drip thing going on. Obviously, you have to go to a clinic and have someone administer an IV, but if you are administering intramuscularly, I think you can do it three or four times a week, 100, 200 milligrams and get pretty good results. I've seen good result and I'll talk about how I have actually enhanced my results with some of the other things we're going to talk Um, you know, as always, there's a certain contention of people that are never going to inject themselves.

12:38 That's cool. I understand that, but it's just kind of a limitation that we're up against. And I would argue this is kind the cornerstone of this whole equation is using injectable NAD, especially as we age, we get into our forties, fifties sixties. Um it just can become impaired and there are things we can do from a lifestyle standpoint to optimize it. But when we look at energy over time, a lot of times it is a missing piece for people because of things in their lifestyle. Now, Let's look at NMN. So this is one of the precursors. It's actually a direct precursor to NAD+. So it's essentially one metabolic step away from becoming NAB.

13:11 This you can take orally or sublingually for better absorption. Um, and it's rapidly converted into any pleasant cells, especially in muscle and other peripheral tissues. So, um, when you talk about benefits, a lot of people are going to love this for its endurance and mitochondrial benefits. The studies are showing the NMN supplementation can enhance aerobic capacity and oxygen utilization during exercise. Typically you have more stamina. In a six week trial on amateur runners, daily NMN significantly improve their oxygen uptake and power output at threshold indicating more efficient energy

13:43 production during intense exercise. And people that use it often report a noticeable increase in stamina and quicker recovery when taking NMM, likely because their muscle mitochondria are producing ATP more effectively. It's also been known to upregulate or two in the muscle, potentially improving muscle repair and insulin sensitivity. I wouldn't say it's, massive in that sense, but it definitely has an effect. And it's more of a systemic NAD plus booster, excluding the brain, which I'm going to talk about within our making a strong choice for the whole body energy

14:13 and metabolic health. So drawbacks. There is kind of this weird uncertainty around the regulatory status of NMN. In the US, it was recently classified as a drug and greedy at making supplement forms harder to obtain. Not impossible. If you're smart, you can get them. And there's websites that sell them, but it is a little bit more, I guess, with that whole thing. It's just less and less easy to find. But there is an ongoing debate on whether NMN enters cells directly or first converts to NR.

14:46 Recent evidence suggests specific transporters allow NNM uptake. Concerns around include cost stability and potential sleep disturbance have taken too late due to this upregulation and energy. And most people are going to take it in the morning because of the endurance and longevity benefits and really affecting exercise performance is what it's known for. Now let's look at NR. And again, all of these are going to be crucial, and don't worry, I'm going get to the dosage and how we're going use all these. But just to keep going with the overview, NR stands for nicotinamide riboside.

15:18 It's another NAD plus precursor, it's actually a form of vitamin B3 that rarely converts to N-AD+. It is often highlighted as more of the brain booster. So NMN, think of it as like the body. And then NR I think is the brain, and also the nervous system. It's smaller and more lyophilic than NMN, potentially allowing it to cross the blood brain barrier and raise NAD plus levels in the brains, in people's brains more efficiently. And this is commonly sold as Niagen, if you've heard of Niogen, the supplement.

15:48 That's like the biggest seller of NR. People that use it report improvements in cognitive function, focus, and even mood. I'll say, yeah, I definitely noticed this when I've used it. It aligns with preclinical studies where NR protected neurons improve memory in the models of Alzheimer's and brain injury. And so in Alzheimer, like mice, NR restored NAD plus levels actually in which help prevent cognitive decline and synaptic loss. Um, and it also supports nerve health. And there's some research in diabetic mice found in our reduced brain inflammation, improved cognitive performance.

16:18 Uh, especially when we look at Alzheimer's, a lot of that is related to inflammation in the brain relative or related insulin signaling, which is kind of like type three diabetes. So, Because NR circulates systemically, it benefits the body too. NR and NMN ultimately feed in the same NAD pool in our body, but a lot of people take it specifically for the neuroprotection and neuroplasticity. And in terms of repair pathways in brain, It protects against the oxidative stress that were up against of the brain which you should also use things like

16:48 creatine and metformin to supplement against. Again, is taken as an oral capsule and has a strong safety profile even at higher doses like one to two grams a day. So the drawbacks not everyone that sees results within our and again This is, I think, dependent on lifestyle. It's dependent NAD plus levels already if you're injecting and other things. Pricing, a lot of these supplements are more expensive. I don't think relative to peptide buyers, they would be that expensive, but just the average person.

17:19 You know, it has more of a cognitive benefit and NMN is going to have more. Of the athletic benefits, if we want both, you are going need both. And obviously the best thing to do would to stack them, which is what we're going talk about once we get into the dosage now. The less covered one, but I think perhaps one of the biggest players in this whole cycle that we can look at to optimize what's going on is five amino. So if you've been to this world, you probably heard what I've just talked about more or less a few times, again, on videos and podcasts and stuff.

17:50 But where I take a lot of people don't necessarily I wouldn't say not get it right, but they just don't understand how much 5-amino could be a beneficiary player to these things. So 5 amino is basically what is known as an NNMT inhibitor. Let's explain what that means. It works a little bit different than these NAD precursors. Instead of supplying N80 plus or supporting blocks, 5 amino inhibits the nicotinamide N-methyltransferase enzyme that consumes N 80 plus precursor.

18:22 NNMT and fat and other tissues can break down nicotinamide vitamin B3 into a usable form, effectively draining the raw material for NAD plus production. Excuse me, I said usable. I meant to say unusable form. And by blocking NMNT, 5-amino prevents this NID destroying pathway and frees up more N, M, N to be recycled into N80 plus. So this results in an increase in cellular NED plus levels and a revved up metabolism. Now, this is where I think Again, what does everybody want? Fat loss and muscle gain.

18:53 When we look at fat loss or muscle game, this is where we're going to get the most bang for our buck out of all these things we are using. Studies in obese mice showed remarkable effects. Five amino given in just over 11 days boosted N80 plus in fat cells, leading to a 5% loss of body weight, but more importantly, a 35% reduction in white fat mass without changing any food take. Treated mice burn more fat, evidenced by a smaller fat cells on 30% drop in blood cholesterol levels, purely through metabolic enhancement that came from the five amino, which obviously makes it as attractive as a body recomp agent.

19:27 So it essentially turns up the body's fat burning furnace by enlisting the NAD plus powered energy pathways. And so this is one that a lot of people use to break through weight loss plateaus, especially in the presence of a GLP-1 when you're kind of up against that. That's a question I get all the time. Like I'm taking for his appetite. I've taken. freaking 15 milligrams of church appetite a week, and I can't keep the weight loss. What do I do? Well, a lot of times we're down regulating these pathways because of the stress we put on the body. And so again, when we look at fat loss, there's multiple pathways that can go into it. This NAD plus pathway is one that a lotta people are just not even utilizing at all, especially in the presence of something like a high GLP-1 dose,

20:05 which could cause more stress that's impairing the NADA pathway along the way. It really helps break weight loss, it's obviously going to improve insulin sensitivity and amplify the effects of diet and exercise on post-production. As always, I would say these things work the best in the presence of exercise and diet. I think specifically with five amino, like in my studies, you saw they weren't changing food intake, they were not necessarily doing anything different, but again, strength training, cardio, controlling your calories that has a long way to go in a lot of these things.

20:42 So of the five amino is definitely the most research chemical, most gray area thing. It's not mainstream, it's often obtained obviously from research, chemical companies. We have limited human data on optimal dose, long-term safety. Now I'll get into my lab rat dosing for this, so don't worry. But some users are going to experience mild effects like headaches or jitters due to metabolic changes, which obviously when we have a powerful metabolic agent, That's just going to happen. I've made videos on that explaining why some of these weird things happen in this metabolic shift, dehydration, jitters,

21:14 all those things. We can use taurine and other agents like that to help. It's going boost our metabolism and ultimately too, I know this sounds stupid, but it is something we have to pay attention to because When we have energy through the roof and we're hitting all these pathways, we've got to be careful of overtraining. And even if you feel really good, you've gotta pay attention to things like your HRV and stress levels to really have an idea of what's going on. Then we the fat burning effects. So it mimics exercise-induced fat burn.

21:44 I think a lot of people want to take that and then they're just like, oh, well, I don't have to exercise because it's doing all of these things. That's not true, like I said. 5-amino is a very potent tool to prevent AD plus depletion and turbocharged fat oxidation and it's best used in cycles for targeted fat loss or metabolic interventions. This wouldn't be one that I use all the time, the same way that i would use AD Plus injectable and the precursors all time which I would still cycle a little bit and we'll talk about. Let's look at, before we get into dosing, I just wanted to talk about some measurable benefits of NAD+.

22:17 So there's growing body of evidence because it's becoming something that is very studied and there are a lot of studies going on about it, but there is measurable performance improvements. So when we sustain or elevate NED+, you empower cells to produce more energy and repair themselves more effectively. Let us look some of the benefits. First, we're going to have enhanced endurance and VO2 max. So for athletes, this is huge. When we jack NAD plus up, it improves mitochondrial efficiency and ATP production, which is going translate to better aerobic or cardiovascular performance.

22:48 In one randomized trial, middle-aged runners who supplemented with NMN saw significant increase in oxygen uptake during exercise. And so while their VO 2 max didn't change dramatically in six weeks, their muscles were utilizing oxygen more effectively to generate energy, for performance purposes is going to help. 2019 crossover study in older adults found that NAD plus supplementation improved exercise performance and reduced the biomarkers of oxidative stress from that exercise, performance. So again, exercise is great, but again it's one of those things we have to keep it in check, right?

23:22 So we haven't too much creates oxidator stress, then downregulates all these pathways that leads to more harm than good. A lot of athletes that use it describe, you know, an extra interval or maintain high intensity longer when their AD plus levels are topped up. I definitely know using injectable NAD plus intramuscularly as a pre-workout. My cardio is better and definitely, I wouldn't say I'm like stronger, but the ability to do more reps is there. So any plus means a higher cellular work output before hitting the wall.

23:52 Azure mitochondria have abundant coenzyme to keep electron transport and ATP synthesis humming. So let's look at this idea of mitochondrial function and ATP production. And this is really cool in lieu of the players like SLU and MOTC and SS31 we have. NAD plus is very important to mitochondrial health, so no surprise. When we boost it, it can revitalize mitochondria health again, alongside some of these peptides that we're already using too. Also too, It allows us to get lower doses out of those so we can sustain them for the long term and we don't necessarily have to create a bunch of oxidative

24:26 stress from overloading the mitochondrion. Raising NAD plus triggers mitochondrial biogenesis and efficiency improvements partly via the sirtuin 1 PGC 1 alpha pathways that promote new mitochondria function. It also has measured outcomes when we look at ATP levels in tissue and greater muscle endurance. In heart muscle cells, for example, extra NAD plus led to increased ATP output and improved function under stress.

24:57 There was also a published review that summarized NED plus restoration leads to improvement of mitochondrial function and increased ATP production. And for biohackers, Yeah, we're going to get better sports performance out of it, but also more day-to-day energy and less mid-afternoon fatigue, which I can definitely attest to. And I think a lot of people, when they start hormones, they feel really good. So let's just take testosterone optimization, for instance. They feel good, and then three, six, maybe 12 months in, starting to be like, oh man, I just don't have the same energy in that same honeymoon phase that

25:28 I had. And a lot of times there could be multiple things going on there. But I think the NAD cycle is kind of being exposed in that sense that meaning that like we're getting these benefits from hormones, but there's still a critical component that's missing for a lotta people as it relates to their energy levels. So let's look at cognitive protection. We talked a little bit about this with but anal studies suggest that boosting NAD can enhance cognitive performance.

25:58 For instance, mice given an NR or NMN show improved memory and maze test. N80 plus fuels CERT1 in neurons, which linked to the growth of new neuronal connections, protection against neurodegeneration. A recent study in rats with cerebral ischemia found that raising N 80 plus improved learning and reduced neurono damage. NAD plus deletion is often associated with cognitive fiber, so maintaining NADA plus may help preserve brain power with aging. Again, this is a tool in the toolkit alongside some more hormones and stuff.

26:29 A lot of users of the precursors report more mental clarity or faster thinking as a benefit. So while human trials are still catching up, it's clear that a well-fed brain in terms of NAAD is primed for better focus, memory and retention. And there's some early clinical data indicating that NAD plus improves cognitive fatigue. One trial showed that the NAH supplementation reduced mental fatigue scores in subjects over 12 weeks. So pretty interesting. Faster muscle recovery, I think anyone that trains regularly can jump on board with this. But also when we reduce inflammation, that's just going to make us better for the long term.

27:03 N80 plus can speed up recovery by activating sirtuins and orchestrate muscle regeneration and by boosting cellular repair of DNA and proteins. In injuries or post-workout stress, higher N 80 plus levels help muscle cells produce energy and mobilize the gene programs needed to repair fibers more rapidly. So just a brief aside, anyone that has an injury or like an area that's really, really sore, I think could benefit from this alongside BPC, TB 500, GHK, PEG, MGF, and all those things. This would be something that you're supplementing with as well.

27:34 It also has anti-inflammatory effects. So it serves as a co-factor for enzymes that tamp down excessive inflammation and it fuels immune cells to help with inflammatory responses. One noted effect of NAD IV therapy in athletes is reduced muscle soreness and quicker return to baseline after exhaustive events like competitions and games we talked about earlier. At the molecular level, it drives the activity of CERT1 and CERT3 which deacetylate and modulate inflammatory pathways and also helps regenerate glutathione as an antioxidant thereby cutting down oxidative inflammation, which again, we can pair this with glutothione to make it work that much better.

28:11 So an athlete that is replenishing with an ADP plus is likely to experience less muscle soreness or DOMS and bounce back faster, which allows them to obviously train more frequently without accumulating as much fatigue or oxidative stress. Now to everybody's point, fat oxidation, metabolic enhancement, what are we going to get out of optimize NAD with this. So the body metabolism tends to shift into higher gear when we have more NID, we burn more efficiently. Ultimately, it comes down to getting better gas mileage out of our body. Is it directly burning fat?

28:41 No, but it's helping get more gas out out what we're doing and also to alongside GLP-1 peptides alongside these things. it's going to help us, especially in the presence of a caloric deficit. So NED plus dependent enzymes are more needed for beta oxidation, which is the process of burning fatty acids for energy. When we boost it, it can increase the rate of which fat is stored. Remember what we said about five amino, 5% one body weight loss in 11 days on those mice from enhanced fat burn. Some human trials with NR showed modest reduction of blood pressure and cholesterol and improved muscle insulin sensitivity,

29:14 especially in older metabolic-impaired subjects. Again, that's the core tenet of everything I talk about is that insulin-sensitivity. It also interacts with AMPK, which is a metabolic switch with ample NA plus cells more readily activate AMP. So it's kind of an indirect AMP K activator. leading to increased fat oxidation and mitochondrial biogenesis. For people that just want to drop that last bit of fatty fat or improve body composition, it also can help, again, improve the gas mileage, and improve everything you're already doing. And you'll notice that weight loss comes easier.

29:46 You have less tendency to gain fat and more stable energy levels. uh, Cincinnati helps process glucose with less insulin. So, um, it obviously has noted effects there. Again, I think it's one of those things you're not going to necessarily get shredded from just hitting this pathway with the things we're talking about. But when you were using a microdiss of a GOP one, a little bit of an SLU, uh little of, of uh a growth hormone peptide or growth from on itself, hormone optimization, desiccated thyroid, that's where it really shines. Now everyone's favorite part, And if you click to the timestamps to be here, uh, good on you for skipping over some of the boring stuff,

30:21 but, um, best entity plus stacking. So what is it going to look like when we stack all these things and how are we going do it specifically? So I kind of codified these into different stacks. This would be, should be a daily maintenance stack of, the easy thing to do. Right. You don't want to inject. Don't have to, do any of that stuff. What's the, easy to. There's just kind, of they are running in the background. This would be NR or NMN every morning alongside other longevity molecules. So we would take 300 milligrams of NmN in the morning.

30:52 You could pair it with something like a resveratrol, which can leverage the increase in a plus and perhaps a booster of NAT like TMG, trimelaglycine. Some prefer 250 to 500 milligrams Nr in morning for brain and body and reserve Nmn for more of the performance enhancement boost for their body. So the idea here is to keep NAD slightly elevated every day, which over time supports DNA repair, metabolic health and energy. Again, in this case, we're just taking the precursors that are going to slightly bump up an AD. It's not going anything to make superhuman or super physiologically energy speaking wise.

31:27 And it's very subtle, so you might not feel it. You probably won't feel. I don't really feel when I'm not doing some of these other things, but under the hood is keeping ourselves younger. biologically ready to perform. So again, this is more of like the under the hood stack, just kind of, like what I'm running in the base ground, you know, background baseline among all these other things. Let's get into a little bit more, of the high performance protocol. This would be starting with the cornerstone of an NAD IV or an intramuscular injection. You could do a 500 milligram ivory drip or a 100 milligram intra muscular shot at the start of a high demand period.

32:00 For instance, when I am training harder, I will do 100 milligrams of NADA plus intravascular pre-workout. makes it work out a lot better. And I kind of noticed the effect probably for another 24 to 36 hours. Especially if I'm training in the morning, it can be something that's pretty taxing on my nervous system if i'm going hard. I don't notice a drop off as much in afternoon where I feel like I want to take a nap because I trained so hard earlier in And then on top of this, you would do daily NMN 500 to 1000 milligrams for physical endurance, and then daily NR 300 milligrams, for cognitive support.

32:30 So again, this can be someone that is probably like myself that, is much more interested in a high performance. The dual precursor approach allows our muscles and neurons to have plenty NAD plus, And you can also throw in CoQ10 and PQQ. They're not NED boosters per se, but they support mitochondrial electron transport. and compliment NAD plus's role in ATP production. You could also look at things like methylene blue at five to 10 milligrams if you wanted to. I also like L-carnitine with an AD plus precursors to enhance fat utilization for energy.

33:04 That works really well. And the high performance action also include hydration electrolytes as NED won't help if your dehydrated. Again, I think I can't hammer that point home enough. in the sense that when we're hydrated, all these things are going to work better and you shouldn't drink water, you should drink with electrolytes because a lot of times you can drink, but you're not necessarily going get the electrolyte if it's dead water. So when you have this protocol that use pop over results, so you feel much more of a high gear capacity, whether it is intense training or just something

33:36 where you are very engaged cognitively that you need your brain to be firing up all cylinders. That would be kind of the high performance stack. Now the next one is going to be the fat loss stack. So this is where we bring in five amino. This wouldn't be something you do all the time, but for four to six weeks periods when we're really trying to get shredded, this where you would add it in. What we do is a four-to-six week cycle where I take five-amino injections or capsules. If you're doing the capsules, I would start with 50 milligrams per day.

34:07 I get much, much more benefit out of the injections, which you can find at reputable research companies that I will not name here, but you could find on the email list. Um, like 500 micrograms, uh, injected sub Q every day, so basically that means if you get a 10 milligram bottle, you'd put two milliliters of water into it and then you do 10 units. So I'll, can slow that down and put it in reverse. If we want 10 milligrams bottle of injectable five amino, we put 2 milliliter of into it, and then you would do 10 units for 500 micrograms per day.

34:37 And that's what I would Do every day, usually first thing in the morning. So I'd also take NR or NMN at 300 or 500 milligrams to ensure ample N80 plus precursors are available. Then you could also add in, I definitely added metformin, then probably a microdose of a GLP-1 like red trutide. to help with glucose management. And again, it's kind of helping get the most gas mileage out of all those peptides. Um, and then, you know, when we couple five amino with NMN, It can yield a potent increase in resting metabolism and fat oxidation.

35:10 You might notice your resting heart rate and your HRV might go, a skew for a little bit. That's totally okay. Cause that happens when you're going through a metabolic shift. This is the way if you want to really drop significant body fat percentage in a month and you'll notice much more muscle. I definitely notice that it definitely seems like help with muscle retention too also in caloric deficit. But basically you're turning your body into a fat burning furnace by fueling the NNR NAD plus pathways and removing the damper on the flame by blocking

35:46 NMT via five aminos. So I would not recommend that you run this all the time. And the thing with five amino too is you are typically going to build up a tolerance to it after four, six or eight weeks, depending on your experience with it. You know, like the longer you've used it, typically the quicker you build a bit tolerance. But if you cycle it on four to six weeks on, four or six week off for fat loss, it works really, really good. And then you can kind of go back more into the maintenance routine. So what would I do to prevent desensitization? So I like a five days on two days off cycle.

36:17 With all of these things, we don't want to use them every single day all the time. Five days, two day off with everything is really. You could also do a monthly cycling approach so we can do NAD plus IV therapy once a month, NMN daily for three weeks and then take one week off the end. And then we can alternate also between NR and NMM to engage different pathways. So that's a good strategy as well. Then obviously to maintain lifestyle NAD boosters like exercise and fasting, even during supplement breaks. I think that that is another thing I haven't mentioned yet, is how important fasting can be to help with all of this and optimize what we are doing.

36:50 From a supplementation standpoint. So how do we maximize from a lifestyle standpoint? One, we can now actually directly measure NAD+. I wouldn't recommend you do it unless you have unlimited funds because it can be really expensive. But we could also look at indirect biomarkers. Um, they're out there and then also insulin sensitivity. I think that's like the cheapest way to measure it. Uh, but we look at lifestyle, uh, fasting, exercise and cold exposure along with quality sleep and, a diet rich in NAD plus precursors is going to be huge.

37:22 Obviously the diet is more just like eat whole clean food. But fasting induces NAMPT, which raises NAD plus levels and activates CERT-1. Cardio and resistance training upregulates NADA, so always be doing those. Cold exposure definitely does, even if it's just a cold shower, you know, one time a day or a few times a week. Quality sleep obviously is huge. I think the biggest thing that we can do to avoid NDA plus killers is stop alcohol. There's one thing you can Depletion in the body is to stop drinking alcohol because that's one of the biggest things that people experience.

37:55 Again, that is why people that drink a lot have such good benefits from N80 plus because they are depleting N 80 plus so much. But if you look at people who don't drink, typically they don t need to replenish as much as someone who is deplenishing all the time. Uh, chronic stress, obviously too, I would probably be guilty of this myself is when we are stressing the body out, whether it's through training or work or whatever it is, um, that's going to have an effect, you know, and then also overtraining can do it. And then poor sleep and high sugar diets also, uh, Deplete a plus so kind of the common sense things, but just be aware of it Now does that mean we use 80

38:34 plus to like overcome these things that we're doing to ourself No But everyone has like exposure to life all of us it's some in shape or form probably is exposed to one of these Things more than you know We would like to be and we can use an 80-plus supplementation to address them and help prevent the the you know, downside of them. So what does the future of NAD plus look like? So there's things kind of on the forefront. One's called NRH, which is dihydro nicotinamide riboside. And there is another one called NMNH which has reduced NN.

39:07 Their next generation precursors seem to have astonishing potency. seen any of those, there may be some out there that you can buy now, but they can raise NAD plus levels much faster and higher than the equivalent doses of NR or NMN, which would be pretty cool, especially if they're oral compounds that we don't have to inject because you're always going to have higher compliance rate with those. They're still in the research phases, but look out for them. They could become available to biohackers in coming years, offering a new tier of performance enhancement without the caveat of,

39:39 with a caveat, excuse me, of ensuring that too much of a good thing doesn't trigger unwanted effects like excessive inflammation. As one study noted, NRH spiked an 80 plus of immune cells, and also promoted inflammatory gene activation. I think one thing to talk about in relation to this, because I haven't mentioned it yet, it actually just crossed my brain, is entity plus in relationship to cancer. So a lot of people say, well, I wouldn't want to do this when. I have cancer because it could potentially give more ATP to the tumor cells.

40:10 And that could be true. So I would just say for people that are working through that life experience, I wouldn't necessarily go gung ho on all these things. Because again, there's other pathways that I've addressed that relate to insulin sensitivity in regard to healing the cancer before I go to NAD+. But just be aware of that. Another frontier that is out there is this idea of the CD38 and PARP inhibitors.

40:42 They come from plant-derived compounds like a better Apigenon analog aimed at shielding any plus from excessive consumption. So CD 38 inhibitor, in particular exciting by inhibiting CD-38, CD38 basically increases with age and chews up NAD+, and we could potentially, just by inhibiting that, restore youthful NAHD levels without even taking precursors. That's pretty cool and something that's kind of on the forefront. Keratin and Apigenin are known CD 38 inhibitors, and when people take these, it's actually helping.

41:14 This is a whole NAAD plus cycle again. And so in the near future, we may see a combined supplement includes an NAD plus precursor like CD38 and a PARP inhibitor together for a one, two punch. So that should be pretty cool. And it's, you know, being worked on as we speak. There's also this idea of gene therapy. so there are things in gene, therapy that are upregulate in a MPT, which causes our body to maintain high needed plus on its own. trials on mice have shown that by tweaking NAD plus related genes, we can extend lifespan, improved health span.

41:48 So it's not too far fetched in a decade or two, We might undergo gene therapies alongside like folistatin and some of these other things to permanently boost N.A.D plus metabolism, which would be pretty cool. But again, it all related to like living and optimize, right? Eating clean, fasting, training, doing cardio, resistance training. All those things are important. And we can also use red light therapy, sauna, and nootropics to help all of the things that we talk about with NAD. Again, it's more, the cycle is not just dependent on the supplementation of NADA.

42:21 It's relative to all these things. And will probably see something like a genetic profile and blood work to dictate whether NR or NMN is better for you, what dose and what add ons are out there. Um, and ultimately NAD plus it's just a smart strategy to recharge yourselves. Now, in summary, I put together a little chart of what I would do. So if you want, you can screenshot this, but you see the daily maintenance. I'm just going to take 500 milligrams of NMN, 300 milligrams NR. Not going worry about the injectable or the five amino. And you use that continuously, You know, even if he wanted to cycle five days on two days off, he could do that for high performance,

42:56 500 to a thousand milligrams, NNM, three hundred milligrams. and then 100 milligrams of intramuscularly injected in 80 plus two to three times per week, or you could do the IV once monthly. No five amino, and this would be during high intensity training or mentally demanding periods, which we all experience. It's best for peak performance and endurance. For fat loss, I'm going to do 300 to 500 milligrams NMN. Remember that's more of the body precursor that works really well. I going do 100mg two, three, times a week of an 80-plus injectable intra muscular or 500mgs once a monthly, And then five amino,

43:30 you can take the capsules at 50 milligrams a day, split into two doses. Um, or you're going to do 50 mg a morning, 50mg the evening. Or my personal preference is do the injectable five-amino at 500 micrograms per day. And you would do this for four to six weeks and then cycle off at least four or six. Weeks. And then, um, just to prevent desensitization, we could just cycle on five days on two days off with the NMN at 500 milligrams, same thing at 300 milligrams for the NR five-days on, two-day off. And we can do the injectable NAD plus intramuscularly two to three times per week or 500mg once monthly.

44:06 and again, We could potentially do this for three weeks and then take one week off and this can prevent any down-regulation of any plus pathways. and ensure long-term effectiveness. So there you have it. You can take a screenshot of that if you want, just for the ultimate stacking protocol. I know some people just want to see that, so that's totally cool. But that is it for slides. And that the entire NAD Plus cycle explained. Hopefully that was helpful and informative, you guys. But let me know your feedback. I'd love to hear if you like these more longer-involved videos.

44:39 This was honestly more really came from my own desire to learn and understand. And as I did, I was like, oh, this would be a good video. So hopefully that's helpful to you guys. Let's say from personal experience, the most that you will, quote unquote, feel from this is when you add in the 5-amino because of what it's doing to the NMMT pathway. Obviously, in our good baseline, probably not going to feel anything. intramuscular injections, probably you're going to feel more from that side of things. And then when we add in the five amino alongside all those things, it works really well.

45:09 You could use all of those independently and probably still get some benefit. But when have them all together, that's when it worked best. So hopefully that helped with you guys. Let me know your feedback and thoughts on this one. I know it was a little bit of a deviation from the normal peptide video, but I'd love to hear what you think. If you want me to do more longer ones like this, I think this will end up being around like 45 minutes or so. I appreciate you guys much love again as always to close out every thank you from the bottom of my heart. It truly is a joy and passion and I am super privileged to get to do what I do every day and bring this information to you.

45:43 I cannot thank guys enough and hopefully that reflects in my work but I just receive all the gratitude from you in whatever shape or form it is that you help support the channel. That's the point now where I can't, you know, talk one-on-one with everyone, but just know that I am extremely grateful, and I'm so excited about the things coming in the future, this year and beyond. So, appreciate you guys, much love, And I will talk to you in next one. Peace.