Why 5 Amino 1MQ Is Your Secret Weapon for Fat Loss & Longevity

0:09 Hey everybody, this is Hunter Williams. I hope you're doing amazing wherever you are at in the world. Today's video is going to be kind of an updated video, but also a little bit of a new one. And I'm going be going into five amino one MQ. So yes, five Amino has been around for, it seems like forever now, probably six or seven years. As I remember when I first heard about it and started using it. But recently, I would say really in the last year it's come on the scene, but I'd say in like the past six months I've played on and off with the injectable

0:39 version that is now making the rounds in a lot of research companies. on their websites now and I really like it. And I definitely like compared to the oral version definitely seems to be more efficacious and does not seem to have the same sort of tolerance buildup that the world version seemed to at least in my case and many other cases of people that I've spoken with. So today I'm going to kind of do an updated and expanded version of my five amino video. Cause at that time when I made that one, injectable may have been out, but it wasn't something that had personally used. I wanted to update and expand just my thoughts on it, And also talk about something too that I have been playing around with,

1:13 which is actually injecting five amino with NAD to see how that does. And it seems to be working really well so far. So probably not a super long video today, but I think valuable nonetheless. If you want, as always, check out the Peptide Cheat Sheet. That link will be down in the description of all my videos. Also too, if you love geeking out on these topics and want a very supportive, amazing community of other people that are like-minded and love to do so as well. Check out Fully Optimized Health. That link is always in the description. And I just love that community. I love the people there. They are my people for sure.

1:43 So if you want to meet amazing other like minded people and also too, we do live Q and A's every Tuesday night where you can join in on those and ask all of your awesome questions. Without further ado, I'm going to share my screen and today we're going learn a little bit more about five amino. All right, I am Hunter Williams and today is the updated 2025 version of 5-amino and we're also going to talk a little bit more about the injectable version. So what is it? So I'm not gonna try to pronounce 5 amino 1MQ but it's 5 amino 1 methylquinolinium.

2:16 I guess I just did, but is a small molecule compound that has gained a lot of attention in the biohacking research, longevity space for its unique metabolic effects and some of its anti-aging benefits too. It is often lumped in with the peptide conversation, again, because most research chemical companies sell it, but is not a peptid hormone but rather a derivative of the quinoline molecule. Hopefully I'm pronouncing that right. But it was originally developed by researchers as an analog of one MQ to improve potency against a specific metabolic enzyme and its mechanism centers

2:48 on inhibiting the enzyme called nicotinamide in methyl transferase, otherwise known as NNMT, which plays a key role in fat metabolism and energy homeostasis. So let's look at the mechanism of how it works and some of where it comes from. This NNMT basically methylates nicaminotide, which is vitamin B3, into one methyl nicamidotides, MNA, using S-adenosyl methionine, otherwise known as SAM. So a lot of big words, but it basically works as the methyl donor.

3:22 It blocks NMNT's activity, so it prevents the conversion of nicamenotid into MNa, allowing it to recycle into NAD plus via the salvage pathway. Now I promise that's going to make sense when we kind of tie this all together, but what this leads to is an intracellular N AD plus increase. So by doing so, it elevates N A D plus levels and preserves S A M levels in cells having wide-ranging metabolic impacts due to the essential roles in energy production and DNA repair. So essentially what we're doing by blocking that enzyme is we are going to boost NAD.

3:56 This kind of removes a metabolic break. NMT slows down fat breakdown and is often upregulated in fat tissue of obese individuals and animals. So again, whether it's the chicken or the egg, who knows, but when we look at obese people and obese animals, typically this enzyme is pretty high and it is upregulated, the activity of it, it up-regulates. So by inhibiting this enzymes, 5-amino boost cellular NAD+, revving up energy expenditure and reducing fat storage signals in the body.

4:27 And we can look at the substitution on the quinolium scaffold made 5-amino about 10 times more potent at inhibiting NNMT than just the 1MQ molecule. So 5 amino is a modification off of that. But when we look its origins, it emerged from academia. from research into NMT as a drug target for obesity and metabolic diseases. And scientists at the university of Texas medical branch identified five amino as lead probe inhibitor of NNM T in the late 2010. So again,

4:58 only like six or seven years old to my knowledge. But early in vitro studies showed 5-amino had high cell permeability and selectivity for NNMT without affecting other similar enzymes significantly. And researchers tested it in animal models as proof of concept that NNT inhibitor based on its promising properties and preclinical results with 5 amino sparked interest in using it to modulate metabolism for therapeutic or biohacking purposes, but mainly for fat loss. Now let's look at what we at least know some of the research so far in terms of benefits.

5:31 So obviously accelerated fat loss, that's what everybody loves it for. But like I said, there's going to be some more there. In diet induced obese mice, treatment with five amino caused a significant reduction in body weight and body fat compared to controls. Obese mice given five-amino over just 11 days lost about 5% of their body without eating less food. And treated mice had approximately 35% smaller fat depots and fat cell size than untreated obese mice, specifically shrinking white fat tissue. And then it also enhanced their fat burning.

6:02 So it combats obesity by enhancing fat-burning rather than suppressing appetite, a la GLP-1s, which do a little bit more than suppressing appetite. But during these trials, food intake remained unchanged and they lost that. So pretty cool there. Obviously as part of a healthy diet and lifestyle, most people will want to control the amount of food they eat. I'm not saying you want it to nix your calories completely, but We can also look at improved metabolic markers, so alongside fat loss, five amino yield, better metabolic health markers in animals.

6:32 So treated mice showed lower plasma cholesterol levels around a 30% reduction, which is nothing to sneeze at, and improved insulin sensitivity relative to controls. Notably, I think what's cool about this is when we look side effects, you can't say something has no side effect relative other medications or Peptides out there is very, very low. So the benefits occur without signs of toxicity or behavioral side effects in the short term, and this indicates the weight loss was due to genuine metabolic modulation, not because the animals got sick or ate less.

7:02 And so by boosting NAD plus and energy expenditure in adipose tissue specifically, five amino essentially help reprogram the metabolism of obese mice to a healthier state, which is pretty cool. Now, what I do like about it too, and this is where we get in the conversation of pairing this alongside GOP1s. And again, I love to talk about the multimodal pathway approach to fat loss. We don't want to take 10 milligrams of trisapatite or red tritide per week. You want do a low dose and we want add in some of these other peptides and small molecules to be able to approach fatloss from a bunch of different angles.

7:36 You don't win the game by scoring seven touchdowns. Sometimes you can win. The game, by a scoring, seven touch downs, but sometimes you only need to score three touchdown because if you play defense and you place special teams, the outcome goes in your favor. Sorry for the football analogy. I love doing football analogies. As if he followed me, you probably know. But again, we don' want to just have a star offense. We want have to start offense and defense in special. Teams and a good coaching staff and good support staff, and all those things, because that goes into winning. And when we're winning the fat loss battle, that's what we want. To do is approach it from different pathways.

8:06 We can look at muscle mass and strength. So, although fat loss is often accompanied by muscle loss with many dietary drugs, a la the GLP-1s, NNMT inhibition may avoid that pitfall. 5-amino increases NAD+, activating pathways tied to muscle maintenance, and potentially sparing lean muscle during weight loss. So recent studies show that 5-amino can enhance muscle performance, improve muscle endurance and recovery, especially in aging individuals. And 5 amino may play a role in improving muscle and endurance recovery which can provide a benefit to fat loss.

8:37 Again, the benefit of fat lost without moving muscle mass. This is something I personally will attest to and I'll go into my personal experience as we go through this, but it definitely enhances energy and exercise capacity. So many people experimenting with it report a boost in metabolic drive, higher energy levels and faster workout recovery, which makes sense given its mechanism of elevating NAD plus and SAM to turbocharge cellular energy pathways. People, again, notice more of a metabolic drive. And I think if you look into the bodybuilding world now, because a lot of the people that are wising up to this, a lotta people will use this in their

9:11 last few weeks of prep, typically which is kind of, the death knell of them because they're so depleted, to help drive energy alongside things like SLU and methylene blue. But I this again is an alternative pathway that we can throw into those to kinda help in those extreme phases of fat loss. but in mice five amino had in groups. treated groups, excuse me, had increased energy expenditure, essentially burning more calories as heat and activity. And this is where we get into the longevity support. So 5-amino has attracted a lot of interest in longevity world due to its impact on molecules like NAD.

9:45 NADA plus boosters such as NR or NMN are known in the anti-aging community for their cellular health benefits. By inhibiting NNMT, 5 amino raises NNAD plus levels in tissues, potentially activating sirtuins and other longevity pathways. It's interesting There's so much of this conversation around NR and NMN and an NAD push IVs. But 5-amino kind of gets left out of the conversation a lot of times, and I understand it's because it is a research molecule, but I think it as important a player as any of those in that whole NADA cycle optimization process.

10:20 So maintaining high SAM levels may support proper DNA methylation and polyamine metabolism processes which are linked to aging. And then by enhancing metabolic fitness, obviously that's the goal, reducing visceral fat, enhancing insulin sensitivity, and increasing NAD+. It indirectly supports longevity and health span. Again, I think you could make the argument, Taylor and I were actually having this conversation the other night that, yeah, love testosterone, but insulin is the one hormone in the body that kind of regulates everything. And if you control insulin in The right way, you can control aging, control inflammation, You control disease, Control all those things by controlling insulin,

10:56 sensitivity. So, researchers suggest NNMT inhibitors may synergize with NAD plus precursors to address age-related metabolic issues, and I would definitely concur with that. So what I did, again, I wanted to speak more to the injectable side of things, because that's where I've been having a lot of success relative to oral, is talk about the difference. When we look at oral bioavailability of 5-amino, you can use the oral. They work good. You'll probably notice something. I know I didn't, but it just seemed to kind of build up a tolerance after 46 weeks.

11:26 But from what we can see, there's about 38% absorbability or absorption of the oral form. So you need higher doses around 50 to 150 milligrams daily or what are often used to ensure The compounds reach circulation, whereas the injectable, it's near 100% bioavailability. It's usually via sub-q injection. So the body absorbs the entire dose. And because it is going directly into the buddy, we're looking at 500 micrograms to one milligram daily, is what I personally have experimented with and found.

11:57 I don't know that there's anybody else that's talked about the injection dosing and frequency. That's why I wanted to make this video. But I, personally, find 500 to 1 milligrams tends to be a good amount. When we look at the onset of action, obviously the oral is going to have a slower onset, so it may take one to two hours after ingestion for you to feel anything, especially if you're taking a pre-workout. But obviously if your doing it injected, you are going feel it pretty much right away. And I notice a stronger and more immediate boost in energy levels when I take this right the way as opposed to the injection as oppose to orals.

12:27 Now, obviously, dosing convenience is very easy to swallow a pill. It's very to sell a pillow to people. More people will adjust the pill, then we'll take an injection. And then the injection is obviously less convenient. You've got to carry around needles or even a little bit better. Maybe you're better off and you have pins like I do that I put it in. But it is something that just comes more with the territory of injecting. it's more of a a pain in the butt, no pun intended. Now, feedback wise, I personally will say I like the injections much better.

12:57 It's not that the orals don't work, but you really have to do a higher dose and it becomes cost prohibitive. The injections I think are a little bit more cost friendly when we talk about the dosing and what you can get out of them, especially relative to the outcome. And then storage, obviously they're Capsules are capsules. You don't have to worry about it. But with the injections, you do have. To worry. About refrigerating it so when you travel, that's something you gotta be conscious of because believe it or not, more hotels don' have refrigerators than you would think. Most of them do, but a lot of em don. So just something to be cautious of. Now, I want to talk about injecting alongside NAD+.

13:30 And again, a few people are talking about the inject 5-amino and I think fewer people have even thought to inject it with an 80+. But this is a theory I had that I wanted to test out on myself and it seemed to go well so far. So I was kind of thinking, okay, 5 amino suppresses NMT, raises cellular NID. That's great. We get a benefit from it. But what if we could inject five amino alongside in 80? What would the result be? So let's look at this. You're kind of ejecting it from two, you're approaching the energy equation from 2 different angles.

14:00 So when we inject NAD+, and I like around 10 milligrams in the presence of 5-amino, because again, I'll explain that in a second, but it's helping with the metabolization of N80+, how it is getting chewed up. But injecting NAD floods the interstitial space with the coenzyme itself. So some of that NAd plus is taken up directly by the cells via transporters, instantly raising intracellular Nad plus concentration, which is good. However, exogenous Nads plus can overcome acute dips in Nd plus that occur with heavy exercise, stress, or metabolic dysfunction,

14:32 providing a quick top-up for sirtuins, PARPs, and other ND plus-dependent enzymes, Now, let's look at slowing the NAD plus strain from within. So under normal conditions, nicaminotide, the breakdown product of NADA plus is methylated by NNMT into one methyl nicotinamide which permanently removes it from the entity salvage pathway. By injecting one milligram of five amino, you block NMNT activity so more nicotenamides is recycled back into NNAD plus rather than being wasted.

15:05 And sometimes people don't get a response from NMT plus, and this is why. So because NMMT uses S-adenosyl methionine, otherwise known as SAM like we talked about earlier, to methylate nicotinamide, inhibiting it also spares SAM, supporting DNA and RNA methylation and polyamine synthesis, which are themselves important for cellular repair and mitochondrial health. Now I promise I'm going to explain what that means in simple terms. So when we do this, it leads to multiple synergies. So we have a maximized NAD plus pool, meaning that we get exogenous and endogenous production.

15:37 And so you're both adding N80 plus directly and preventing its unnecessary diversion via these other pathways, resulting in a larger net increase in tissue in 80 plus levels than either strategy alone, whether that's just using an AD plus by itself or whether we're just five amino by self. We also have the activation of sirtuins. So srtuins, specifically SIRT1 through 7, are NAD plus dependent deacetylases that regulate mitochondrial biogenesis, antioxidant defenses,

16:08 and DNA repair. A higher NADA plus to NAH ratio potentiates sörtuin activity, improving mitochondria function, stress resilience, metabolic flexibility. So with more NAD plus available via suppressing the NNMT enzyme, cells can more efficiently shuttle electrons through dehydrogenases, reducing reactive oxygen species accumulation and supporting better energy production. So we actually get more of an antioxidant effect from the plus when we use 5-amino with it.

16:38 And so by conserving SAM, which is the universal methyl donor, you maintain a healthy epigenetic regulation and polyamide synthesis which contributes to cellular growth, autophagy and longevity pathways. So When we look at this from a practicality standpoint, what is the best of both worlds? I think if you were splitting hairs, you could get into this. I'll talk about what I do in a second. But many users inject NAD plus first to rapidly raise levels, and then they'll follow with five amino around 15 to 30 minutes later to ensure nicotinamide

17:08 generated by any turnover is shunted back in the salvage rather than methylated. That's being a little anal, but if we were looking at the metabolization of these, that's what you would extrapolate to. And so you could do subcutaneous of both. It's well tolerated, allows slow absorption. Intramuscular can deliver a slightly faster peak, but maybe more uncomfortable. Now I've done both with this. I do feel it a little bit more with the intramuscular, but then it's like, do you want to have to do an intra-muscular shot with a larger needle, or can you just do a sub-Q shot, with your pen? Both work. Intra muscular, a bit better, But I think you're good either way.

17:40 And so you could do two to three times per week for NAD+, depending on that. Or you can do daily or every other day, five amino, Or You could just take them at the same time together. and that's what I did. So you obviously want monitor your energy, sleep, and then just keep an eye on liver enzymes. methylation markers like comocysteine. If you're using a high dose NAD or prolonged five amino use, because that's something that could creep up on you. I don't think it's really something you have to lose sleep over, but it just, when you look at all the potential things that can happen, what would be the case? So here's exactly what I would do if I wanted to take the stack and combine it together.

18:14 So I'm going to have five, you know, typically those are sold in 10 milligram vials. And so I'd put two MLS of water into that five Amino. Now it's five milligrams per milliliter. And to get one milligram, I'm going to inject 20 units. Then I take five ML of backwater because typically NAD plus and 500 milligrams comes in a 10 ML vial. So I would put five MLS of water into my 500 milligram vile of an 80 plus, and then I am going use 10 units of that for 10 milligrams because it is a hundred milligrams for milliliters. If I want 10 milligrams, it will be 10 unit.

18:44 Together I will inject twenty units, five amino and ten units NED plus. Sub-Q or shallow IM, you could do that into the muscles that you're training. If you prefer, sub-q is probably a little bit easier and you just don't have to worry about with as much pain as if you were doing an IM with a bigger needle. And for me personally, it's okay to combine these in the same syringe. So that's what I do. You don' have too, but if want to you can, You could also potentially mix those in same big vial if wanted to. Mix up your 5-amino, mix up NAD, put them together in a same vile. Just make sure you do your math right.

19:14 For me, that's what I do. And I inject them together and I just pull into the same syringe and inject from there. So pretty simple, but I think you can stand to benefit from using these together. Like I said, if you were being really anal retentive, you could do that schedule that I mentioned before. But again, I'd just inject together in the morning. I'll typically do my training days and then maybe one or two of my fasted cardio days. That is it for the slides. And that is my comprehensive overview of 5-amino, now updated to include the injectable version. I personally am a bigger fan of the injected version than the oral version, both work, but I do think you will get more benefit out of injectible version

19:50 as with most things. Orals are great. You get good oral efficacy, But the injections are better. From a thyroid loss standpoint, I'm really lean right now. Probably sitting somewhere around 7 or 8% body fat. I wouldn't say it's like stripping fat off of me, but I do think for someone that needs to go from maybe 15% body fat down to 10% Body Fat, this would have a pretty good effect. Again, that's not my specific use case for this, But I think it is something that you could definitely use to do that. And again, it s another pathway that we can use attack fat loss.

20:22 Like I was talking about earlier, We have all these different modalities for fat loss. We don't want to just put the foot down on the gas on one pathway. we want it to approach it from a holistic, multimodal lens and look at, okay, what's working and then how can we minimize the doses of things to kind of get the best of all possible worlds as it relates to fatloss because there are so many different areas that we can attack as relates that. So hopefully this is helpful to you guys. If you have experience with the injectable I know it's relatively new in the space, so there's not a lot of people talking about it. Leave him the comments just so people can see, hey, this worked for me, or hey this was worthless.

20:54 Hunter doesn't know what he's talking, about because I think that's helpful to the community. Obviously we have our private communities, but for all the people in comments and stuff that just watch the videos, I do think it is helpful the more you can share your personal experience with those things go, and it totally cool. I'm very, very open to not. banning or deleting comments as long as you're not harmful to other people out there. So thank you guys so much. Again, check out the links down in the description for the email list and all that good stuff. I really, really love, appreciate, and just have the utmost gratitude for everyone that supports the channel and whatever form of fashion.

21:24 And I always say, hopefully that comes across in material that I create. Thank you, guys, so, much I love each and every one of you and I will talk to you in an next one. Peace.